Journal: Journal of microbiology and biotechnology
Article Title: Anti-Inflammatory Efficacy of Human-Derived Streptococcus salivarius on Periodontopathogen-Induced Inflammation.
doi: 10.4014/jmb.2302.02002
Figure Lengend Snippet: Fig. 6. Binding inhibition of LPS or LTA of periodontopathogens to CD14 and LBP by S. salivarius LTA. EIA plates were coated with rhCD14 (A-C) and rhLBP (D-F). The plates were incubated with biotinylated P. gingivalis LPS (100 ng/ml), T. forsythia LPS (100 ng/ml), and F. alocis LTA (100 ng/ml) in the presence or the absence of S. salivarius G7 LTA and type strain LTA. The bound LPS and LTA were detected using HRP-labelled streptavidin and TMB solution. The experiments were performed three times in duplicate, and data are represented as means and standard deviations. Asterisk (*) indicates statistically significant differences compared with non-treated group (p < 0.05).
Article Snippet: Recombinant human LBP (rhLBP, R&D Systems) and human CD14 (rhCD14, R&D Systems) were dissolved in DPBS at a concentration of 2 μg/ml and were added into suitable Ab-coated well.
Techniques: Binding Assay, Inhibition, Incubation